• Improved lane analysis background compensation algorithm.
• Lane analysis supports dynamic and user defined background compensation. Enable it via the JustTLC properties accessible from the main menu.
• Background compensation can be locked or dynamic during band editing.
• Live feedback in graph overlay that shows lane and band editing. Is turned on via the Imaging pane on Plate Analysis page.
• Improved project export with better distribution of plate images.
• Improved customization of project export for with settings for headers and graphs.
• One-click open or locate of exported project after creation.
• Significant memory optimization during project export to support megapixel images.
• Making it possible to start dragging a crop region outside of plate image.
• Detecting and notifying JustTLC user when Windows account access rights prevents logging and managing of users.
• Fixed bug during lane analysis when doing bit depth compensation affecting quatification results.
• Fixed bug causing lane analysis to be incorrect sometimes when applying higher order noise filtering.
• Fixed bug in project export that caused lane profile graph to become corrupt sometimes.
• Fixed bug in project export that caused image color inversion to be unstable sometimes.

• Ensuring that access level is controlling ability to add, edit or delete users.
• Adding more verbose logging when editing a user.

• Extending support for JPEG rotation information, to make sure that images are rotated correctly in JustTLC.

• Making sure that all lane profiles are included when exporting a project to a document.
• Updating plate navigation when adding or removing plates from a project.

• Adding possibility to reset license from login form.
• Improving usability when registering a license key.

• Suppot for high dpi screens making the UI more crisp and sharp.
• Run JustTLC in batch mode with command line arguments.
• JustTLC can analyze up to 50 plates in one batch without a single click in the UI.
• Export analysis result to pdf or word documents.
• Support for lane analysis with automatic band count detection in batch mode.
• Locate project by clicking on the project name in the statusbar at the bottom of the application.

• Batch mode example:
• Windows key + R then type in cmd and click on the "Ok" button.
• In the command window do:
• > cd "C:\Program Files (x86)\Sweday\JustTLC\"

• For lane analysis with automatic band detection:
• > Sweday.TLC.Desktop.exe Plate 1 "Sample Project\Plate.jpg" Development Standard Analysis LaneAnalysis LaneCount 3 BandSizePercentageOfLane 10 BandVolumePercentageOfMax 5

• For lane analysis:
• > Sweday.TLC.Desktop.exe Plate 1 "Sample Project\Plate.jpg" Development Standard Analysis LaneAnalysis LaneCount 3 BandCountInTotal 30

• For spot analysis:
• > Sweday.TLC.Desktop.exe Plate 2 "Sample Project\Plate1.tif" "Sample Project\Plate2.tif" Development Standard Analysis SpotAnalysis SpotCount 24

• Including writing result to file:
• > Sweday.TLC.Desktop.exe Plate 2 "Sample Project\Plate1.tif" "Sample Project\Plate2.tif" Development Standard Analysis SpotAnalysis SpotCount 24 OutputFileName "%UserProfile%\Documents\JustTLC_results.pdf" OutputFileMode Create

For a complete list of commands and more examples please refer to the User Guide.

• Added project description to output during printing of project overview page.
• Fixed bug in JustTLC file association that sometimes made it impossible to double click on .tlc files.
• Fixed bug in interactive Rf value rendering that prevented accurate positioning of overlays during editing on plate analysis page.

• Updated to .Net Framework 4.0 for optimal performance on Windows 8.
• Added the possibility to export 16-bit images as 8-bit images.

• Fixed bug that caused lane profile graph to sometimes fail to render.

• New amazing image analysis algorithms for lane samples supporting bent lanes.
• Automatic matching of lanes and bands across plates for simple and straightforward data comparison.
• Wonderful illustration of lanes for direct comparison using lane profiles.
• Powerful editing capabilities for lanes and bands with support for cloning.
• New automatic and semiautomatic Rf calculations for both spots and lanes.
• Improved project overview information with one click location on disk of included images.
• Enhanced overlay graph by making horizontal and vertical lines alternate between white and black.
• Added support for .png image file format.
• Added support for undo/redo notes editing and increased undo/redo history.
• Data table on Plate Comparison page displays sum of selected volumes.
• Several bug fixes.

• Added possibility to increase or decrease number of spots to detect without extending the dropdown list.
• Significantly improved Project Overview load time when switching between views.
• Fixed bug in recent projects list that caused unsaved projects to be added to the list.
• Fixed bug that caused project to sometimes become unsaved when the Plate Analysis page is opened.

• Fixed bug in login authentication that prevented some registered users from entering.
• Fixed bug in recent projects list causing list update even if opening a project was canceled.

• Fixed bug in background estimation.
• Fixed bug in image zooming when minimizing JustTLC.

• New gorgeous GUI on all views and toolbars.
• Region of interest to control analysis algorithms without needing to crop images.
• Support for user defined color to grayscale conversion.
• Support for displaying negative colored images (images with bright spots on dark background).
• Contrast slider to dynamically alter contrast for displayed images on the fly.
• Possibility to invert colors on Plate Analysis page (Imaging panel).
• Spot cloning to give new spots identical area and contour.
• Custom definition of background region (for each spot) replacing automatic background subtraction.
• Undo/Redo spot editing.
• Undo/Redo plate comparison settings changes.
• Short cut keys to e.g. save (Ctrl+S)
• Multiple spot selection by dragging rectangle over spots or clicking in spot table.
• Select/unselect spots by clicking inside their contours.
• Edited spot contours and spot clones may overlap neighbouring spots.
• Simultaneous highlight of spots in both spot table, graph and image.
• Increasing number of spot series from six to eighteen.
• Graph reflects sorting in spot table. Sort spots and graph is updated.
• Quick navigation between different spot series by drawing horizontal lines during editing.
• Improved graph rendering.
• Improved data interaction on plate comparison page by displaying both graphs and spots simultaneously.
• Restrictions are optional. Change it on the properties page and deactivate login page.
• More interactive overlayed intensity graph that follows mouse pointer.
• Project templates on start page.
• Possibility to export color to grayscale converted images to .tif files on disk.
• Export images includes image preview with option for inverting colors.
• Tips and tricks on start page instead of getting started text.

• Improved support for 64-bit machines.
• Improved support for unicode languages.

• Improved background estimation robustness when having few spots.

• Significantly improved spot detection.
• Improved spot quantification robustness.
• Increase spot detection and quantification speed.
• Added full support for regulations in FDA 21 CFR Part 11, e.g. user management, data signing, logfiles and encryption.
• Reduced memory consumption during spot detection and quantification.
• Improved background compensation.
• Optional background compensation.
• Improved software start up time.
• Added new Notes column in spot data table with smart autocomplete mechanism.
• Added presentation of intensity percentage during mouse over on a plate.
• Added intensity graph overlayed on plate for a horizontal or vertical slice.

• Added support for 48-bit and 64-bit color tif images.
• Increased maximum possible spots to 300 per plate.
• Improved project loading when project or images have been moved from original location.
• Made graphs clickable on Plate Comparison page.
• Fixed color invert when adding a new plate to a color inverted project.
• Improved image loading feedback when format is not supported.
• Fixed minor bug in quantification. Markers were not evenly distributed.

• Added support for scaled spot volume as displayed volume. Setting plate normalized as optional.
• Improved graph by making grid line spacing use even numbers.
• Fixed line contour editing bug.

• Added organization of data to enable grouping and averaging of plates.
• Updated layout on Plate Comparison page to support grouping of plates.
• Added support for arbitrary shape on edited contours using lines.
• Increased robustness of plate volume normalization by taking the mean of the median and two neighboring spots.
• Improved graph look-and-feel by adding a 15% margin to the y-axis, filled circles in line graphs and average lines in bar graphs.
• Improved layout on Plate Analysis page to increase plate image size.
• Added missing spots in spot series as having zero volume.
• Fixed mouse coordinate bug. Incorrect coordinates were displayed when moving mouse over plate image.
• Fixed status bar bug. Project name was not updated when saved with another name (Save As...).
• Fixed bug in contour extractor, which caused failure to find contour in rare cases.

• Improved rendering of images and overlays.
• Adding support for images with zero dpi.
• Upgrading included PDF viewer to FoxitReader 3.0

• Adding project export functionality to CSV and Excel formats.
• Adding support for multiple spot series assignment using drag rectangle.
• Improving cursor cross visibility during editing of spots and spot series.
• Replacing html help with PDF help to improve accessibility of information.
• Adding option for installing PDF viewer during installation.
• Fixed bug on plate comparison page using same graph y-scale.

• Improving evaluation version support further. Now, extended language cultures are also included in the 2.4.1 fix.

• Improving evaluation version support so that everyone can evaluate JustTLC.
• Fixing so that major and minor version upgrades (2.4 -> 2.5) are notified but not build (2.4.0 -> 2.4.1). To check for new build versions use the help menu.

• Increased analysis throughput by improving navigation between plates.
• Extended support for large scale projects.
• Adding support for printing of e.g. graphs, plates and spot data.
• Adding automatic check for upgrades during start up.
• Fixed resizing of plate display so that it stays the same when switching between plates.
• Fixed rendering bug when zooming.
• Fixed clear spots bug that caused spots to still render after they had been deleted.
• Fixed bug in spot detection that did not reset spot volume if desired spots > actual.

• Adding possibility to invert plates in Project Overview.
• Improved background estimation when editing spot contours.
• Improving spot contour editing so that only affected spot's volume is changed.
• License type now displayed on startup.

• GUI updates to sync software with website.
• Licensing made easier.

• Added tutorial to help section.
• Improved highlight of selected plate in Project Overview.
• Headers in each control view added.
• Improved project creation.
• Fixed bug in spot data grid.

• Improved data comparison using spot data grid and graphs.
• Simplified user interface for improved interaction.
• New spot volume normalization methods.
• Zooming.
• Spot contour editing.
• Plate analysis control view updated.
• Excel export extended with real spot volume.

• Improved spot quantification.
• Spot detection speed up.
• More flexible GUI.

• Sample project updated.
• Help pages extended.

• Dynamic GUI layout based on plate height and width.
• Improved project creation.

• Concept of project introduced.
• Improved usage of spot series.
• Project overview added.
• Plate comparison added.
• Start page added.
• Graphs supported.
• Help added.
• Improved spot quantification.

• Improved background estimation.
• Contrast enhancement.
• Plate overlays.
• Spot higlighting.

• Spot volume normalization.
• Excel export.
• Licensing.
• Improved spot detection and quantification.